CE-MS for metabolomics: advancing performance and detection sensitivity

Leiden Repository

CE-MS for metabolomics: advancing performance and detection sensitivity

Type: Doctoral Thesis
Title: CE-MS for metabolomics: advancing performance and detection sensitivity
Author: Zhang, W.
Issue Date: 2020-03-31
Keywords: CE-MS
Sheathless interface
Sheath liquid interface
HepG2 cells
Metabolomics
Nanosafety
Ultra-small biological samples
Abstract: The major and ultimate aim of metabolomics is to obtain an answer to a specific biological or clinical question. For that, many strategies have been applied in this field, including NMR and MS-based approaches. CE-MS is one of the techniques in this field but remains underappreciated because of issues such as compromised sensitivity and poor reproducibility. In this thesis, we proposed standardized protocols for CE-MS studies using a sheathless interface and applied them in the metabolic analysis of ultra-small biological samples, such as low numbers of HepG2 cells. Another CE-MS technique used in this thesis is the traditional sheath liquid CE-MS, and it was successfully used in the metabolic profiling of plasma samples. We also introduced CE-MS with both interfaces in the field of nanosciences with our efforts in revealing the adsorption characteristics of polar metabolites to the surface of nanomaterials. This work clearly demonstrates that CE-MS can be reliably performed for metabolomics studies with acceptable repeatability. The use of sheathless CE-MS interface enables us to have in-depth profiling for ultra-small samples, and may become a powerful tool in the emerging field of single-cell analysis. 
Promotor: Supervisor: Hankemeier T. Co-Supervisor: Ramautar R.
Faculty: Faculty of Science
University: Leiden University
Uri: urn:isbn:9789083055213
Handle: http://hdl.handle.net/1887/87241
 

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