Connecting dots between natural and artificial photosynthesis : magnetic resonance studies on light harvesting and the water oxidation reaction centre

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Connecting dots between natural and artificial photosynthesis : magnetic resonance studies on light harvesting and the water oxidation reaction centre

Type: Doctoral Thesis
Title: Connecting dots between natural and artificial photosynthesis : magnetic resonance studies on light harvesting and the water oxidation reaction centre
Author: Sunku, K.
Issue Date: 2019-12-13
Keywords: Photosynthesis
NPQ
Solid state NMR
EPR
Three flash flow cell
Abstract: Decentralized plug and play systems for energy production are the future picture of our society. Artificial photosynthetic systems are used for this purpose. These systems are inspired by natural photosynthesis.Chapter 2 illustrates the detailed construction of a three-flash flow cell to study light induced biological reactions, specifically the water oxidation reaction of Photosystem II. This instrument is capable of producing the S2+, S3+ and S0n intermediate states of the Kok cycle. I confirmed these by EPR experiments.In chapter 3, I study the mechanism of the major LHC II involved in conformational switching from light harvesting to the photoprotective state, in which excess light is dissipated as heat. I use MAS NMR as a non-invasive method to understand the structure and environment around Arg residues in Chlamydomonas reinhardtii LHC II. In this approach the Arg amino acid is selectively labeled with 13C isotopesChapter 4 studies the structure of self-aggregated semi synthetic Zn 31 amino chlorin that forms syn-anti parallel stacks according to the solid state NMR data. The small change of removing 33 methyl group of Zn 31 aminomethyl chlorin has significant effect on self-assembly. Finally, chapter 5 provides an outlook on future research.
Promotor: Supervisor: Groot H.J.M. de Co-Supervisor: Pandit A.
Faculty: Faculty of Science
University: Leiden University
Uri: urn:isbn:9789492597366
Handle: http://hdl.handle.net/1887/81787
 

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application/pdf Chapter 1 254.0Kb View/Open
application/pdf Chapter 2 604.9Kb View/Open
application/pdf Chapter 3 723.6Kb View/Open Full text at publisher site
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application/pdf Chapter 5 66.76Kb View/Open
application/pdf Summary in English 536.9Kb View/Open
application/pdf Summary in Dutch 369.2Kb View/Open
application/pdf Curriculum Vitae_Acknowledgements_Publications 952.1Kb View/Open
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