Design, synthesis and application of paramagnetic NMR ptobes for protein strucutre studies

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Design, synthesis and application of paramagnetic NMR ptobes for protein strucutre studies

Type: Doctoral Thesis
Title: Design, synthesis and application of paramagnetic NMR ptobes for protein strucutre studies
Author: Miao, Q.
Issue Date: 2019-11-28
Keywords: Paramagnetic NMR probes
proteins
PCS
PRE
cyclen
TraNP1/2
HMDOTA
Abstract: The main subject of this thesis is the design and synthesis of paramagnetic molecules for protein studies with NMR and EPR spectroscopy. With the development of paraNMR and DEER experiments, synthetic paramagnetic centers are becoming popular. About half of the current paramagnetic probes were described in the past five years, reflecting this popularity. Several of them improved the stability and rigidity of the probes, mainly by introducing novel attachment groups forming thioether and triazole linkers.1–3 Such linkages help to extend the application of the probes to in-cell measurements.4,5 Some small probes were designed for 3d-block metal ions as well, but these probes show low metal ion binding affinity.6,7 The research described in this thesis contributed to the development of paramagnetic probes. Hereafter, the properties of these new probes are discussed, and some general conclusions and prospects are given.
Promotor: Supervisor: Ubbink M. Co-Supervisor: Overhand M.
Faculty: Faculty of Science
University: Leiden University
Handle: http://hdl.handle.net/1887/81086
 

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