Imaging of alkyne-functionalized ruthenium complexes for photoactivated chemotherapy

Leiden Repository

Imaging of alkyne-functionalized ruthenium complexes for photoactivated chemotherapy

Type: Doctoral Thesis
Title: Imaging of alkyne-functionalized ruthenium complexes for photoactivated chemotherapy
Author: Busemann, A.
Issue Date: 2019-10-01
Keywords: Anticancer complexes
Ruthenium polypyridyl complexes
Click chemistry
Protein interaction
Photoactivated chemotherapy
Fluorophore labelling
Abstract: In photoactivated chemotherapy (PACT), a biologically active compound is caged by a light-cleavable protecting group. Light irradiation leads to the release of the active species which can then interact with the cell environment to induce cell death. In recent years, ruthenium polypyridyl complexes have proved to be promising candidates for PACT. To obtain more insight in the mode of action of these potential anticancer complexes, we explored their intracellular distribution. Post-treatment fluorophore labelling via click chemistry allowed for the visualization of the non-emissive ruthenium polypyridyl complexes in fixed cells.
Promotor: Supervisor: Bonnet S., Bouwman E.
Faculty: Science
University: Leiden
Uri: urn:isbn:9879463755238
Handle: http://hdl.handle.net/1887/78473
 

Files in this item

Description Size View
application/pdf Full Text 13.42Mb Under embargo until 2021-04-01
application/pdf Cover 1.100Mb View/Open
application/pdf Title Page_Contents 346.8Kb View/Open
application/pdf Chapter 01 913.8Kb View/Open
application/pdf Chapter 02 3.574Mb Under embargo until 2021-04-01
application/pdf Chapter 03 805.6Kb Under embargo until 2021-04-01
application/pdf Chapter 04 817.5Kb Under embargo until 2021-04-01
application/pdf Chapter 05 814.5Kb Under embargo until 2021-04-01
application/pdf Chapter 06_Summary 572.1Kb View/Open
application/pdf Appendices 7.921Mb View/Open
application/pdf Summary_in Dutch 356.3Kb View/Open
application/pdf Summary_in German 491.5Kb View/Open
application/pdf Publications_Acknowledgements_CV 355.9Kb View/Open

This item appears in the following Collection(s)