Identifying small RNAs derived from maternal- and somatic-type rRNAs in zebrafish development

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Identifying small RNAs derived from maternal- and somatic-type rRNAs in zebrafish development

Type: Article / Letter to editor
Title: Identifying small RNAs derived from maternal- and somatic-type rRNAs in zebrafish development
Author: Locati, M.D.Pagano, J.F.B.Abdullah, F.Ensink, W.A.Olst, M. vanLeeuwen, S. vanNehrdich, U.Spaink, H.P.Rauwerda, H.Jonker, M.J.Dekker, R.J.Breit, T.M.
Journal Title: Genome
Issue: 5
Volume: 61
Start Page: 371
End Page: 378
Pages: 8
Issue Date: 2018
Keywords: ribosomal RNA
small-rRNA derived
embryogenesis
zebrafish
development
Abstract: rRNAs are non-coding RNAs present in all prokaryotes and eukaryotes. In eukaryotes there are four rRNAs: 18S, 5.8S, 28S, originating from a common precursor (45S), and 5S. We have recently discovered the existence of two distinct developmental types of rRNA: a maternal-type, present in eggs and a somatic-type, expressed in adult tissues. Lately, next-generation sequencing has allowed the discovery of new small-RNAs deriving from longer non-coding RNAs, including small-RNAs from rRNAs (srRNAs). Here, we systemically investigated srRNAs of maternal- or somatic-type 18S, 5.8S, 28S, with small-RNAseq from many zebrafish developmental stages. We identified new srRNAs for each rRNA. For 5.8S, we found srRNA consisting of the 5′ or 3′ halves, with only the latter having different sequence for the maternal- and somatic-types. For 18S, we discovered 21 nt srRNA from the 5′ end of the 18S rRNA with a striking resemblance to microRNAs; as it is likely processed from a stem-loop precursor and present in human and mouse Argonaute-complexed small-RNA. For 28S, an abundant 80 nt srRNA from the 3′ end of the 28S rRNA was found. The expression levels during embryogenesis of these srRNA indicate they are not generated from rRNA degradation and might have a role in the zebrafish development.
Handle: http://hdl.handle.net/1887/69528
 

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