Clinical glycomics of antibodies by mass spectometry

Leiden Repository

Clinical glycomics of antibodies by mass spectometry

Type: Doctoral Thesis
Title: Clinical glycomics of antibodies by mass spectometry
Author: Haan, N. de
Issue Date: 2019-02-14
Keywords: Glycomics
Antibody
MALDI-TOF-MS
LC-MS
Sialic acids
Saliva
Plasma
Murine glycosylation
Biomarker
Abstract: In this thesis, methods were developed, and antibody glycosylation was characterized in order to further the clinical application of antibody glycosylation analysis. New mass spectrometric workflows were introduced in Chapters 2 and 8. Chapter 7 showed the differential characteristics of murine IgG glycosylation of different strains and highlighted the profound differences between humans and mice, with regard to IgG glycosylation. Chapters 4 and 8 showed the use of controlled human situations to study the regulatory mechanisms of IgG and IgA glycosylation. Finally, Chapters 3, 5 and 6, identified glycosidic differences with specified (patho)physiological conditions, which might be exploited for patient stratification in the future.
Promotor: Supervisor: Wuhrer M. Co-Supervisor: Falck D.
Faculty: Medicine / Leiden University Medical Center (LUMC)
University: Leiden
Uri: urn:isbn:9789463752367
Handle: http://hdl.handle.net/1887/68328
 

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application/pdf Chapter 01 1.006Mb View/Open
application/pdf Chapter 02 2.484Mb View/Open
application/pdf Chapter 03 1.675Mb View/Open
application/pdf Chapter 04 1.598Mb View/Open
application/pdf Chapter 05 1.536Mb View/Open
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application/pdf Chapter 08 1.988Mb View/Open
application/pdf Chapter 09_Discussion and Perspectives 311.0Kb View/Open
application/pdf Addendum 535.7Kb View/Open
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