Enhancing reovirus for use in oncolytic virotherapy

Leiden Repository

Enhancing reovirus for use in oncolytic virotherapy

Type: Doctoral Thesis
Title: Enhancing reovirus for use in oncolytic virotherapy
Author: Kemp, V.
Issue Date: 2019-02-07
Keywords: Reovirus
Oncolytic
Cancer
Autophagy
Transgene
Abstract: Oncolytic reovirus has the natural preference to kill transformed cells while sparing normal cells. Its administration has been shown to be safe in clinical trials, but the anti-cancer efficacy remains to be improved. In this thesis, we discuss several aspects that are important for the design of a potent anti-cancer therapeutic strategy using reovirus. We explored which cellular factors and pathways are important for efficient reovirus replication. Furthermore, we genetically modified the reovirus genome to encode potentially therapeutic transgenes. We tested the potency of these recombinant reoviruses, and describe what we believe is the most promising strategy to move forward. Moreover, we discuss the stability issues that we encountered during the generation of recombinant reoviruses. Finally, we discuss the various challenges and opportunities in how to proceed.
Promotor: Supervisor: Hoeben R.C. Co-Supervisor: Wollenberg D.J.M. van den
Faculty: Medicine / Leiden University Medical Center (LUMC)
University: Leiden
Uri: urn:isbn:9789463324403
Handle: http://hdl.handle.net/1887/68327
 

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application/pdf Full Text 16.84Mb Under embargo until 2020-08-07
application/pdf Cover 3.121Mb View/Open
application/pdf Title Page_Contents 327.1Kb View/Open
application/pdf Chapter 01 569.3Kb View/Open
application/pdf Chapter 02 1.390Mb View/Open Full text at publisher site
application/pdf Chapter 03 5.979Mb View/Open Full text at publisher site
application/pdf Chapter 04 2.593Mb View/Open Full text at publisher site
application/pdf Chapter 05 1.933Mb View/Open Full text at publisher site
application/pdf Chapter 06 3.083Mb Under embargo until 2020-08-07
application/pdf Chapter 07 829.0Kb View/Open
application/pdf CV_Publications_Acknowledgements 1.842Mb View/Open
application/pdf Summary_in Dutch 253.3Kb View/Open
application/pdf Propositions 84.81Kb View/Open

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