Optogenetic investigation of cardiac arrhythmia mechanisms

Leiden Repository

Optogenetic investigation of cardiac arrhythmia mechanisms

Type: Doctoral Thesis
Title: Optogenetic investigation of cardiac arrhythmia mechanisms
Author: Feola, I.
Issue Date: 2018-12-11
Keywords: Arrhythmia
Optogenetics
Atrial fibrillation
Ventricular Arrhythmias
Ablation
Computer-based model.
Abstract: Cardiac arrhythmias are one of the major causes of morbidity and mortality in the world. The clinical management of cardiac arrhythmias relies on several anti-arrhythmic therapies, e.g. drugs, ablation, and device implantation that, unfortunately, are often unspecific, irreversible and/or traumatizing, respectively. Recently, to overcome these side effects, gene therapy has been introduced as an alternative option to treat cardiac arrhythmias by specifically targeting the biological defect. However, gene therapy alone is lacking the possibility to gain precise spatiotemporal and quantitative control over a certain target. Interestingly, more recently, such precise control could be achieved with an innovative strategy, called optogenetics. Here, gene therapy is combined with optics allowing expression of light-activatable proteins that are specifically activate and deactivate by simply turning light on and off, respectively. This now opens new and unique possibilities for cardiac arrhythmias research based on optical modulation of excitability with superb spatiotemporal resolution. Therefore, in the studies presented in this thesis, the unique features of optogenetics were employed for investigating the underlying mechanism of arrhythmia initiation, maintenance, and termination in order to identify novel biological anti-arrhythmic strategies.
Promotor: Supervisor: Schalij M.J. Co-Supervisor: Pijnappels D.A., Vries A.A.F. de
Faculty: Medicine / Leiden University Medical Center (LUMC)
University: Leiden
Uri: urn:isbn:9789461829238
Handle: http://hdl.handle.net/1887/67391
 

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application/pdf Full Text 37.69Mb Under embargo until 2020-06-11
application/pdf Cover 24.42Mb View/Open
application/pdf Title Page_Contents 102.9Kb View/Open
application/pdf Chapter 01 161.2Kb View/Open
application/pdf Chapter 02 3.153Mb View/Open Full text at publisher site
application/pdf Chapter 03 12.76Mb View/Open Full text at publisher site
application/pdf Chapter 03_Appendix 1 108.5Kb View/Open
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application/pdf Chapter 07_Summary 128.8Kb View/Open
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application/pdf Chapter 08_Back matter 181.0Kb View/Open
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