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A day in the life of a germ cell - on gametogenesis and pluripotency
In this thesis, several critical time-points of human germ cell development are addressed.In Chapter 2, we adopted a culture system combining features of human embryonic
stem cells (hESCs) derivation, by supplementation with Activin A, and extended blastocyst culture in vitro to examine lineage segregation in human blastocyst outgrowths. In Chapter 3, migratory and early post-migratory hPGCs were extensively characterized in a rare 4.5 week-old human embryo. In Chapter 4 an extensive characterization of the expression pattern and subcellular
compartmentalization of the PIWIL protein family during human embryonic development and spermatogenesis was performed.Several studies suggest that at some point during the transition from naïve to primed, pluripotency competency for PGC induction is acquired in culture, in parallel with what happens in vivo. In Chapter 5, requirement of BMP-SMAD signalling to keep pluripotency in naïve and ground state culture conditions was...
Show moreIn this thesis, several critical time-points of human germ cell development are addressed.In Chapter 2, we adopted a culture system combining features of human embryonic
stem cells (hESCs) derivation, by supplementation with Activin A, and extended blastocyst culture in vitro to examine lineage segregation in human blastocyst outgrowths. In Chapter 3, migratory and early post-migratory hPGCs were extensively characterized in a rare 4.5 week-old human embryo. In Chapter 4 an extensive characterization of the expression pattern and subcellular
compartmentalization of the PIWIL protein family during human embryonic development and spermatogenesis was performed.Several studies suggest that at some point during the transition from naïve to primed, pluripotency competency for PGC induction is acquired in culture, in parallel with what happens in vivo. In Chapter 5, requirement of BMP-SMAD signalling to keep pluripotency in naïve and ground state culture conditions was analysed.
Show less- All authors
- Gomes Fernandes, M.
- Supervisor
- Mummery, C.L.
- Co-supervisor
- Chuva de Soupa Lopes, S.M.
- Committee
- Goumans, M.J.H.; Pelt, A.M.M. van; Heindryckx, B.
- Qualification
- Doctor (dr.)
- Awarding Institution
- Faculty of Medicine, Leiden Uiversity Medical Center (LUMC) , Leiden University
- Date
- 2018-11-07
- ISBN
- 9789462999961