Quantitative live cell imaging of glucocorticoid receptor dynamics in the nucleus

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Quantitative live cell imaging of glucocorticoid receptor dynamics in the nucleus

Type: Doctoral Thesis
Title: Quantitative live cell imaging of glucocorticoid receptor dynamics in the nucleus
Author: Keizer, V.I.P.
Issue Date: 2018-11-01
Keywords: Glucocorticoid receptor
Single molecule microscopy
Gold nanorods
depth of focus correction
Estrogen receptor
Transcription factor dynamics
Transcription factor nuclear distribution
Abstract: In this thesis, the focus lies on studying glucocorticoid receptor dynamics in living cells with the aim of understanding how this transcription factor finds its DNA target sites to regulate transcription. We have uncovered several GR dynamics states and obtained insight into how the receptor finds its target, which includes repetitive switching between states. In addition, we have made a quantitative description of the static distribution of this transcription factor in the nucleus and compared this to a homologous transcription factor, the estrogen receptor. We have advanced the experimental techniques used to answer this question through development of novel analysis tools. These include a method to correct analytically for observation bias of different diffusion modes in a two dimensional plane. Furthermore, we have investigate the use of an alternative labeling method, namely the gold nanorod. To this end, the behavior and functionalization of gold nanorods was characterized in live cells.
Promotor: Supervisor: Schaaf M.J.M., Noort S.J.T. van
Faculty: Faculty of Science
University: Leiden University
Uri: urn:isbn:9789463324212
Handle: http://hdl.handle.net/1887/66716
 

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