CRISPR/Cas-induced targeted mutagenesis with Agrobacterium mediated protein delivery

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CRISPR/Cas-induced targeted mutagenesis with Agrobacterium mediated protein delivery

Type: Doctoral Thesis
Title: CRISPR/Cas-induced targeted mutagenesis with Agrobacterium mediated protein delivery
Author: Schmitz, D.J.
Issue Date: 2018-09-20
Keywords: Type IV secretion systeml
T4SS
Agrobacterium
CRISPR/Cas
Targete mutagenesis
Protein translocation
Abstract: The RNA guided endonuclease based on the CRISPR/Cas system of Streptococcus pyogenus is a potent new tool for genome engineering in plants. Delivery of this system is usually done by transforming plants (and other organisms) with Agrobacterium tumefaciens which transfers a T-DNA encoding the required genes through its Type IV Secretion System (T4SS). Instead, here we report the delivery of the Cas9 protein of the CRISPR/Cas system of Streptococcus pyogenus through the T4SS of Agrobacterium to Nicotiana benthamiana and Saccharomyces cerevisiae.
Promotor: Supervisor: Hooykaas P.J.J. Co-Supervisor: Pater B.S. de
Faculty: Faculty of Science
University: Leiden University
Handle: http://hdl.handle.net/1887/65634
 

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