Activity-based proteomics of the endocannabinoid system

Leiden Repository

Activity-based proteomics of the endocannabinoid system

Type: Doctoral Thesis
Title: Activity-based proteomics of the endocannabinoid system
Author: Rooden, E.J. van
Issue Date: 2018-09-11
Keywords: Activity-based protein profiling
Proteomics
Endocannabinoid system
Activity-based probes
Niemann-Pick type c
Label free quantification
Quenched activity-based probe
Bio orthogonal chemistry
Abstract: This thesis describes the use of an activity-based proteomics method to study the endocannabinoid system. A protocol for label-free chemical proteomics to measure serine hydrolase activity in mouse tissue is described. This method is used to compare a Niemann-Pick Type C mouse model to healthy mice. Additionally, several novel activity-based probes for the enzymes diacylglycerol lipase alpha and a/b-hydrolase domain containing protein 6 are described.
Promotor: Supervisor: Stelt M. van der, Overkleeft H.S.
Faculty: Faculty of Science
University: Leiden University
Handle: http://hdl.handle.net/1887/65174
 

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