Development of novel strategies to regenerate the human kidney

Leiden Repository

Development of novel strategies to regenerate the human kidney

Type: Doctoral Thesis
Title: Development of novel strategies to regenerate the human kidney
Author: Leuning, D.G.
Issue Date: 2018-07-03
Keywords: Kidney regeneration
Kidney transplantation
Regenerative medicine
Stem cell therapy
Kidney bioengineering
Abstract: Within this thesis several novel strategies to regenerate the human kidney are exploited. The first strategy is to improve kidney function is by mesenchymal stromal cell (MSC) therapy. In chapter 2 the current status of clinical trials with MSC therapy are discussed. In chapter 3 we show an extensive characterization of MSCs derived from human kidney (hkPSCs) compared to bone marrow derived MSCs (bmMSCs) and show that hkPSCs show organotypic expression signatures and functionality. For fluent clinical translation, we developed a clinical grade acceptable standard operation procedure (SOP) (chapter 4). In chapter 5 we show that the cytokine secretion profile of both hkPSCs and bmMSCs was closely related to cell morphology adaptation to culture surface topography and was stromal cell type specific. In chapter 6 we show that not only the kidney cortex but also the kidney capsule contains a stromal cell population. In chapter 7 we report the regeneration of kidney vasculature by repopulating the vascular compartment of human and rat kidney matrices with hiPSC-derived endothelial cells. We show efficient cell delivery, adherence and survival of these endothelial cells as a first, but critical, step towards a human bioengineered kidney.
Promotor: Supervisor: Rabelink A.J., Kooten C. van Co-Supervisor: Engelse M.A.
Faculty: Medicine, Leiden University Medical Center (LUMC)
University: Leiden
Uri: urn:isbn:9789462959712
Handle: http://hdl.handle.net/1887/63486
 

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application/pdf Cover 7.258Mb View/Open
application/pdf Title Pages_Contents 505.5Kb View/Open
application/pdf Chapter 1 1.065Mb View/Open
application/pdf Chapter 2 1.299Mb View/Open Full text at publisher site
application/pdf Chapter 3 15.13Mb View/Open Full text at publisher site
application/pdf Chapter 4 4.757Mb Under embargo until 2019-07-08 Full text at publisher site
application/pdf Chapter 5 5.562Mb View/Open Full text at publisher site
application/pdf Chapter 6 6.414Mb View/Open Full text at publisher site
application/pdf Chapter 7 33.53Mb Under embargo until 2020-01-03
application/pdf Chapter 8 648.5Kb View/Open
application/pdf Chapter 9: Summary in Dutch 708.8Kb View/Open
application/pdf Curriculum Vitae_Publications_Acknowledgements 609.2Kb View/Open
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