Image-based phenotypic screening for breast cancer metastasis drug target discovery

Leiden Repository

Image-based phenotypic screening for breast cancer metastasis drug target discovery

Type: Doctoral Thesis
Title: Image-based phenotypic screening for breast cancer metastasis drug target discovery
Author: Fokkelman, M.
Issue Date: 2017-11-22
Keywords: Cell_migration
Cell_adhesion
Focal_adhesion
Breast_cancer
RNAi_screen
Metastasis microscopy
Abstract: The main aim of this thesis was to unravel the signaling and regulatory networks that drive tumor cell migration during breast cancer metastasis. Understanding how tumor cells migrate, how this process is differentially regulated, and how this highly heterogeneous and plastic behavior is coordinated during metastatic dissemination, will ultimately provide novel insights in therapeutic opportunities. The work presented in this thesis includes different microscopy techniques and analytical tools to study migratory behavior (Chapters 2, 3, 4, and 5), reveals an important role for adhesion GPCRs in cell adhesion and migration (Chapter 3), provides a compendium of genes that drive tumor cell migration in breast cancer (Chapter 4), and investigates the intricate connection between adhesion dynamics, cell migration and contractility (Chapter 5). Collectively, this work provides novel insights in the molecular determinants of tumor cell migration and will help us better understand the signaling landscape that drives the formation of breast cancer metastases.
Promotor: Supervisor: Water B. van der
Faculty: Science
University: Leiden
Uri: urn:isbn:9789402808360
Handle: http://hdl.handle.net/1887/57506
 

Files in this item

Description Size View
application/pdf Full text 10.35Mb Under embargo
application/pdf Title page_Table of contentst 356.2Kb View/Open
application/pdf Chapter 1 Gener ... n and scope of this thesis 1.172Mb View/Open
application/pdf Chapter 2 1.449Mb Under embargo Full text at publisher site
application/pdf Chapter 3 2.055Mb View/Open Full text at publisher site
application/pdf Chapter 4 2.976Mb View/Open
application/pdf Chapter 5 4.590Mb View/Open Full text at publisher site
application/pdf Chapter 6 Summary and discussion 587.3Kb View/Open
application/pdf Summary in Dutch 419.7Kb View/Open
application/pdf List of abbrevi ... lications_Curriculum Vitae 538.1Kb View/Open
application/pdf Propositions 570.5Kb View/Open

This item appears in the following Collection(s)