Association morphologies of amphiphilic polyelectrolyte diblock copolymers

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Association morphologies of amphiphilic polyelectrolyte diblock copolymers

Type: Doctoral Thesis
Title: Association morphologies of amphiphilic polyelectrolyte diblock copolymers
Author: Korobko, Alexander Viktorovitch
Publisher: Leiden Institute of Chemistry, Soft Condensed Matter Group, Faculty of Mathematics and Natural Sciences, Leiden University
Issue Date: 2006-12-12
Keywords: Association
Brushes
Charge
Complexes
Counterion distribution
Diblock copolymer micelles
Gene delivery
Liquid-crystal formation
Phases
Rheology
Small-angle neutron scattering
Star-branched polyelectrolytes
Supercoiled DNA
Abstract: In the first part of this thesis, polyelectrolyte copolymer micelles are studied to elucidate the role of the micelle concentration on the intermicelle correlation and the extension of the polyelectrolyte chains in the coronal layer. With increasing packing fraction the corona shrinks and/or interpenetrates in order to accommodate the micelles in the increasingly crowded volume. It is shown that interpenetration of the polyelectrolyte brushes controls the fluid rheology: the viscosity increases dramatically and the dynamic moduli show the formation of a physical gel. In the second part, we study giant vesicles as a superstructure self-assembled by dsDNA fragments, pUC18 or pEGFP-N1 and oppositely charged cationic diblock copolymer poly(butadiene-b-N-methyl 4-vinyl pyridinium. Under a preparation, the PBd brush collapses and a capsule is formed. The compaction of DNA is shown by the appearance of liquid crystalline textures under crossed polarizers and the increase in fluorescence intensity of labelled DNA. To form vesicles, the capsules are dispersed in aqueous medium supported by an osmotic agent. The integrity of the DNA after encapsulation and subsequent release was confirmed. We demonstrate ``reverse'' transfection of in vitro cultured HeLa cancer cells growing on plasmid-copolymer vesicles by the expressed green fluorescent protein in cultured cells.
Description: Promotores: J.R.C. van der Maarel, J.G.E.M. Fraaije
With summary in Dutch
Faculty: Faculteit der Wiskunde en Natuurwetenschappen
Citation: Korobko, A.V., 2006, Doctoral thesis, Leiden University
Sponsor: NWO
Handle: http://hdl.handle.net/1887/5568
 

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