Magnetic tweezers experiments in low salt conditions and unzipping of double stranded DNA

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Magnetic tweezers experiments in low salt conditions and unzipping of double stranded DNA

Type: Master thesis
Title: Magnetic tweezers experiments in low salt conditions and unzipping of double stranded DNA
Author: Heinsman, Joost
Issue Date: 2017-09-04
Keywords: Magnetic Tweezers
low salt conditions
ionic strenght
drag force
flow cell
diffusion
unzip
G-C content
DNA
chromatin fiber
PEG-DA
Abstract: The structure of chromatin fibers has been researched in different salt conditions with Magnetic tweezers. The drag force on the tether as a result of flowing in a new buffer into the flow cell resulted in structural changes of the chromatin and disassembly of the histone octamer. In a new flow cell design, the drag force was eliminated by changing the buffer conditions by diffusion through a permeable membrane made from PEG-DA 400. Furthermore, double stranded DNA was unzipped using Magnetic tweezers and the position of the melting force was correlated with the G-C content calculated from the sequence.
Supervisor: Noort, John van
Faculty: Faculty of Science
Department: Physics (Master)
Specialisation: Physics
ECTS Credits: 5
Handle: http://hdl.handle.net/1887/52796
 

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