Early events in alpha-synuclein aggregation

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Early events in alpha-synuclein aggregation

Type: Master thesis
Title: Early events in alpha-synuclein aggregation
Author: Vliegenthart, Jan Dirk
Issue Date: 2017-08-31
Keywords: alpha-synuclein aggregation fluorescence FCS
Abstract: Α-synuclein is a small 14 kDa protein, which is believed to play a role in the Parkinson’s disease. The aggregation process of α-synuclein could be the reason for the death of the neural cells in the human brain during the disease. In order to understand this aggregation process, we need to set up a method for measuring the small aggregates of α-synuclein. This report will show the results of burst analysis, Fluorescence Correlation Spectroscopy in solution and in gel. Using the last method, we can find a size dependence on the diffusion coefficient of -1.64. FCS in gel could be performed for finding the early events in α-synuclein aggregation (monomer, dimer and tetramer)
Supervisor: Aartsma, Thijs
Faculty: Faculty of Science
Department: Physics (Master)
Specialisation: MA Physics
ECTS Credits: 3
Handle: http://hdl.handle.net/1887/52786

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