Two-photon luminescence of gold nanorods: applications to single-particle tracking and spectroscopy

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Two-photon luminescence of gold nanorods: applications to single-particle tracking and spectroscopy

Type: Doctoral Thesis
Title: Two-photon luminescence of gold nanorods: applications to single-particle tracking and spectroscopy
Author: Carozza, S.
Journal Title: Casimir PhD Series
Issue Date: 2017-07-04
Keywords: Gold nanorods
Single-particle tracking
Two-photon
Two-photon excitation spectroscopy
Abstract: Gold nanorods are biocompatible nanoparticles that present an excellent two-photon signal that can be used to get high spatial resolution inside living cells. Gold nanorods are photostable and therefore can be followed inside cells for long time, with possible applications as trackers in live cells. We explored the use of gold nanorods as labels for single-particle tracking in live cells, both not functionalized and functionalized with nuclear localization signal for nuclear targeting. For single-particle tracking we used mean square displacement analysis, after characterizing the limitations of this analysis with simulations. We also tested the acquisition of excitation spectra of single gold nanorods for sensing applications.
Promotor: Supervisors: S.J.T. van Noort; T. Schmidt
Faculty: Science
University: Leiden
Uri: urn:isbn:9789085933038
Handle: http://hdl.handle.net/1887/50407
 

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