Biological properties of the oncoproteins E6 and E7 from mucosal and cutaneous HPV types

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Biological properties of the oncoproteins E6 and E7 from mucosal and cutaneous HPV types

Type: Doctoral Thesis
Title: Biological properties of the oncoproteins E6 and E7 from mucosal and cutaneous HPV types
Author: Dong, Wen Liang
Publisher: Department of Medical Microbiology, Faculty of Medicine / Leiden University Medical Center (LUMC), Leiden University
Issue Date: 2006-11-14
Keywords: Cell cycle
Delta Np73
Skin cancer
Abstract: Through epidemiological and biochemical studies the role of mucosal HPV types in human cancer has been confirmed and the molecular mechanisms underlying the transformation process have been elucidated. However, although research on the cutaneous HPV types is progressing fast, many questions remain unanswered regarding their potential role in human skin carcinogenesis.This thesis describes the characterization of the biological properties of E6 and E7 proteins from mucosal and cutaneous HPV types using novel or previously described assays and models. First, we describe the development and use of an in vitro systems to evaluate and quantify the ability of the oncoprotein E7 to associate with pRb (chapter 2). However, we show that this binding although indicative of a high risk HPV type, does not necessarily correlate with in vivo carcinogenicity (chapter 3) and that other in vitro studies are essential to asses the carcinogenicity of any given HPV type. Next we focused on the cutaneous HPV type 38, which displays in vitro transforming properties and appears to be frequently present in NMSC. We show that this cutaneous HPV type can increase the risk for development of skin cancer (chapter 4)and we describe a novel HPV transformation mechanism (chapter 5).
Description: Promotor: W.J.M. Spaan, Co-promotor: M. Tommasino
With summary in Dutch
Faculty: LUMC
Citation: Dong, W.L. , 2006, Doctoral thesis, Leiden University
ISBN: 9085592429

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application/pdf Title page_Table of contents 165.4Kb View/Open
application/pdf Chapter 1 General introduction 1.931Mb View/Open
application/pdf Chapter 2 198.8Kb Under embargo
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application/pdf Chapter 4 773.7Kb Under embargo
application/pdf Chapter 5 238.3Kb Under embargo
application/pdf Supplemental figures Chapter 5 71.38Kb Under embargo
application/pdf Chapter 6 801.0Kb View/Open
application/pdf Summary 124.2Kb View/Open
application/pdf Summary in Dutch 124.9Kb View/Open

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