Typhoid fever : aspects of environment, host and pathogen interaction

Leiden Repository

Typhoid fever : aspects of environment, host and pathogen interaction

Type: Doctoral Thesis
Title: Typhoid fever : aspects of environment, host and pathogen interaction
Author: Ali, Soegianto
Publisher: Department of Infectious Diseases, Faculty of Medicine, Leiden University Medical Center (LUMC), Leiden University
Issue Date: 2006-11-02
Keywords: Typhoid fever
Genetic polymorphisms
Food hygiene
Paratyphoid fever
Cystic Fibrosis
Bacterial strain typing
S.paratyphi A
Abstract: In a surveillance study in Jakarta, Indonesia, 88 typhoid and 26 paratyphoid fever patients were identified by blood culture. Risk factors for transmission of typhoid fever were mainly intra-household factors (poor hand-washing hygiene, recent household contacts), whereas paratyphoid was mainly contracted through street food. In an additional study, street vendors observed poorer hand-washing and food-handling hygiene compared to food handlers in restaurants, and had higher bacterial loads in dishwater. Further host factor-studies in (para)typhoid patients revealed that polymorphisms in genes encoding pro-inflammatory cytokines (IFN-γ, IL1A/B, IL1R1, TNFRSF1A, CASP1 and CRP) were not associated with susceptibility to typhoid fever, and might therefore at most be associated with severity of disease. An association was found of typhoid fever and a polymorphism in the PARK2/PACRG proteasome-mediated protein degradation pathway through ubiquitination, similar to infection with Mycobacterium leprae. Also an association between genotypes in the Cystic Fibrosis Transmembrane Conductance Regulator (the affected protein in Cystic Fibrosis) and susceptibility to typhoid fever was found, suggesting a decreased adherence potential of S. typhi to intestinal mucosal cells with these polymorphisms. Finally, bacterial characteristics were compared by use of AFLP and biochemical/antibiotic susceptibility profiles, showing very homogeneous S. typhi and S. paratyphi A strains circulating in the study area.
Description: Promotor: J.T. van Dissel, Co-Promotores: C. Surjadi, E. van de Vosse, S. Widjaja
With Summary in Dutch
With Summary in Indonesian
Faculty: LUMC
Citation: Ali,S., 2006, Doctoral Thesis, Leiden University
ISBN: 9090210245
Sponsor: KNAW 99-MED-02
Handle: http://hdl.handle.net/1887/4965

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