Activity-based protein profiling of diacylglycerol lipases

Leiden Repository

Activity-based protein profiling of diacylglycerol lipases

Type: Doctoral Thesis
Title: Activity-based protein profiling of diacylglycerol lipases
Author: Baggelaar, M.P.
Issue Date: 2017-04-06
Keywords: Activity-based protein profiling
2-arachidonoylglycerol
Proteomics
Diacylglycerol lipase
Inhibitor
Abstract: Diacylglycerol lipase (DAGL)-α and -β are enzymes responsible for the biosynthesis of the endocannabinoid 2-arachidonoylglycerol. Selective and reversible inhibitors are required to study the function of DAGLs in neuronal cells in an acute and temporal fashion. The work described in this thesis has led to the identification of an activity-based probe (ABP) for DAGL-α. This ABP was instrumental for the identification of a new class of highly selective DAGL inhibitors. These novel inhibitors can be used to investigate the role of the diacylglycerol lipases in metabolic syndrome and neurodegenerative diseases such a Parkinson’s disease, Alzheimer’s disease and Multiple Sclerosis.
Promotor: Supervisor: H.S. Overkleeft, Co-Supervisor: M. van der Stelt
Faculty: Science
University: Leiden
Uri: urn:isbn:9789462995512
Handle: http://hdl.handle.net/1887/48284
 

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application/pdf Chapter 1 418.2Kb View/Open
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application/pdf Chapter 7 1.345Mb Under embargo until 2018-09-06 Full text at publisher site
application/pdf Chapter 8_Summary 977.1Kb View/Open
application/pdf Summary_in Dutch 494.1Kb View/Open
application/pdf List of publications 381.6Kb View/Open
application/pdf Curriculum Vitae 373.5Kb View/Open
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