Glycomic signatures of colorectal cancer

Leiden Repository

Glycomic signatures of colorectal cancer

Type: Doctoral Thesis
Title: Glycomic signatures of colorectal cancer
Author: Holst, S.
Issue Date: 2017-01-24
Keywords: Colorectal cancer
Glycosylation
Glycan
Mass spectrometry
Cell lines
MS-imaging
Abstract: Glycan modifications of proteins and lipids form an integral part of the cell’s outermost layer and an array of ligands, adding a high degree of complexity to the cellular phenotype. While this complexity is an analytical challenge, it also offers a wide range of opportunities for biomarkers and treatment targets. This thesis deals with the analysis of colorectal cancer (CRC)-associated glycomic changes. Current knowledge on CRC-associated glycan changes and their biological role have been reviewed in Chapter 1. In Chapters 2, 3, and 6, we developed novel, high-end methodologies for the glycomic analysis of tissues and cell lines to be able to expand our knowledge on cancer glycomics and to overcome some limitations of current techniques. By applying these new methods, this thesis also covers the characterization of changes in glycosylation in CRC tissues as well as cell lines, thereby contributing to the understanding of CRC biology while identifying cancer-specific signatures underlying CRC development. These signatures can be further explored as potential markers to improve patient care. Additionally, in Chapter 5, we extended our research to pancreatic duct adenocarcinoma and characterized the N-glycome of PDAC cells with different metastatic potential and of a normal pancreatic duct cell line.
Promotor: Supervisors: M. Wuhrer; A.M. Deelder Co-Supervisor: Y. Rombouts
Faculty: Medicine
University: Leiden
Uri: urn:isbn:9789462955622
Handle: http://hdl.handle.net/1887/45594
 

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application/pdf Full text 51.84Mb View/Open
application/pdf Title page_Table of contents 78.18Kb View/Open
application/pdf Chapter 1 General introduction 3.090Mb View/Open Full text at publisher site
application/pdf Chapter 2 2.701Mb View/Open Full text at publisher site
application/pdf Chapter 3 10.50Mb View/Open Full text at publisher site
application/pdf Chapter 4 4.199Mb View/Open
application/pdf Chapter 5 2.958Mb View/Open
application/pdf Chapter 6 25.42Mb View/Open Full text at publisher site
application/pdf Chapter 7 General discussion 1.086Mb View/Open
application/pdf Appendices List ... lications_Acknowledgements 1.986Mb View/Open
application/pdf Propositions 316.9Kb View/Open

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