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Allele specific gene expression on chromosome 7 in human tumorigenesis
The retention of heterozygosity on chromosome 7, we hypothesised, is due to the presence of a set of mono-allelically expressed genes on this chromosome, which are essential for tumor cell survival; so called cell survival genes. Loss of either copy of chromosome 7 would result in a complete loss of expression of those cell survival genes which are exclusively expressed from that particular allele.
We have identified 6 imprinted cell survival genes on chromosome 7, that play a role in chromosome 7 retention in...Show more
Both copy number losses and homozygosity of chromosome 7 are extremely rare events in many tumor types, indicating that the retention of both the maternal and paternal copies of chromosome 7 is essential for the tumor cells. This thesis compiles our research into the driving force that is behind the retention of heterozygosity on chromosome 7.
The retention of heterozygosity on chromosome 7, we hypothesised, is due to the presence of a set of mono-allelically expressed genes on this chromosome, which are essential for tumor cell survival; so called cell survival genes. Loss of either copy of chromosome 7 would result in a complete loss of expression of those cell survival genes which are exclusively expressed from that particular allele.
We have identified 6 imprinted cell survival genes on chromosome 7, that play a role in chromosome 7 retention in thyroid cancer. Additionally, we have analysed allele specific expression on chromosome 7 in a set of novel low passage colorectal cancer cell lines. We also report the extensive characterization, as well as the transcriptome and methylome profiling of these cell lines.
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- All authors
- Boot, A.
- Supervisor
- Morreau, J.
- Co-supervisor
- Wezel, T. van
- Committee
- Maarel, S.M. van der; Janssen, B.; Engeland, M. van; Water, B. van de; Boer, J.M.
- Qualification
- Doctor (dr.)
- Awarding Institution
- Medicine , Leiden University
- Date
- 2017-01-11
- ISBN
- 9789090300436