Virus-host metabolic interactions: using metabolomics to probe oxidative stress, inflammation and systemic immunity

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Virus-host metabolic interactions: using metabolomics to probe oxidative stress, inflammation and systemic immunity

Type: Doctoral Thesis
Title: Virus-host metabolic interactions: using metabolomics to probe oxidative stress, inflammation and systemic immunity
Author: Schoeman, J.C.
Issue Date: 2016-12-20
Keywords: Metabolomics
Viral infection
Immunity
Liquid Chromatography
Mass spectrometry
Metabolism
Abstract: In this thesis, metabolomics is used to study the role of the host-virus interaction on a metabolic level. A special emphasis is directed on the role of inflammation and oxidative stress on the metabolic level, as part of the innate immune response against viral infection. We chose respiratory syncytial virus (RSV) and hepatitis B virus (HBV) as candidate viruses to metabolically study their role in acute respiratory infection and chronic hepatitis B infection. Secondly we also investigated infant metabolic and immunological consequences of in utero exposure to antiretroviral intervention and human immunodeficiency virus (HIV). Collectively, established targeted metabolomics approaches in conjunction with newly developed metabolomics methodologies and complemented with other “omics” techniques, were used to address pertinent questions related to host metabolic functioning and alterations during viral infection. In vitro RSV studies together with in vivo patient based studies relating to chronic HBV infection and in utero exposure too antiretroviral and HIV were used to address these questions. The work is divided into three research parts containing: i. the analytical methodology development work, ii. in vitro based metabolomics and iii. patient based metabolomics.
Promotor: Supervisors: T. Hankemeier; R. Berger Co-Supervisor: R.J. Vreeken
Faculty: Science
University: Leiden
Uri: urn:isbn:9789090300979
Handle: http://hdl.handle.net/1887/45223
 

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application/pdf Acknowledgement ... Vitae_List of publications 218.5Kb View/Open
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