Single-molecule dynamics at variable temperatures

Leiden Repository

Single-molecule dynamics at variable temperatures

Type: Doctoral Thesis
Title: Single-molecule dynamics at variable temperatures
Author: Zondervan, Rob
Publisher: Molecular Nano-Optics and Spins (MoNOS),Leiden Institute of Physics, Faculty of Mathematics and Natural Sciences, Leiden University
Issue Date: 2006-03-16
Keywords: Single Molecules
Optical Microscopy
Cryogenic Temperatures
Rotational Diffusion
Supercooled Liquids
Heterogeneous Dynamics
Local Heating
Temperature Cycles
Abstract: Single-molecule optics has evolved from a specialized variety of optical spectroscopy at low temperatures into a versatile tool to address questions in physics, chemistry, biology, and materials science. In this thesis, the potential of single-molecule (and ensemble) optical microscopy at variable temperatures is demonstrated: Electron transfer has been identified as a crucial step in the photodynamics of organic fluorophores, and long-term memory effects have been discovered in the relaxation dynamics of supercooled glycerol. Additionally, a novel technique, temperature-cycle microscopy of single-molecule dynamics, has been developed. This method aims to increase the observation time in single-molecule experiments at room temperature. The necessary rapid temperature cycles by laser-induced local heating inside a cryostat have been characterized and calibrated.
Description: Promotor: M.Orrit, Co-Promotor: F.Kulzer, Referent: T.Basché
With Summery in Dutch
Casimir PhD Series 2006-03
Faculty: Faculteit der Wiskunde en Natuurwetenschappen
Citation: Zondervan,R., 2006, Doctoral Thesis, Leiden University
ISBN: 9085930103
Sponsor: Stichting FOM, Casimir Research School

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