Quaking promotes monocyte differentiation into pro-atherogenic macrophages by controlling pre-mRNA splicing and gene expression.

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Quaking promotes monocyte differentiation into pro-atherogenic macrophages by controlling pre-mRNA splicing and gene expression.

Type: Article / Letter to editor
Title: Quaking promotes monocyte differentiation into pro-atherogenic macrophages by controlling pre-mRNA splicing and gene expression.
Author: Bruin, R.G. deShiue, L.Prins, J.Boer, H.C. deSingh, A.Fagg, W.S.Gils, J.M. vanDuijs, J.M.Katzman, S.Kraaijeveld, A.O.Böhringer, S.Leung, W.Y.Kielbasa, S.M.Donahue, J..PZande, P.H. van derSijbom, R.Alem, C.M. vanBot, I.Kooten, C. vanJukema, J.W.Esch, H. vanRabelink, T.J.Kazan, H.Biessen, E.A.Ares, M.Zonneveld, A.J. vanVeer, E.P. van der
Journal Title: NATURE COMMUNICATIONS
Issue: 7
Volume: 2016
Start Page: 10846
Issue Date: 2016
Abstract: A hallmark of inflammatory diseases is the excessive recruitment and influx of monocytes to sites of tissue damage and their ensuing differentiation into macrophages. Numerous stimuli are known to induce transcriptional changes associated with macrophage phenotype, but posttranscriptional control of human macrophage differentiation is less well understood. Here we show that expression levels of the RNA-binding protein Quaking (QKI) are low in monocytes and early human atherosclerotic lesions, but are abundant in macrophages of advanced plaques. Depletion of QKI protein impairs monocyte adhesion, migration, differentiation into macrophages and foam cell formation in vitro and in vivo. RNA-seq and microarray analysis of human monocyte and macrophage transcriptomes, including those of a unique QKI haploinsufficient patient, reveal striking changes in QKI-dependent messenger RNA levels and splicing of RNA transcripts. The biological importance of these transcripts and requirement for QKI during differentiation illustrates a central role for QKI in posttranscriptionally guiding macrophage identity and function.
Handle: http://hdl.handle.net/1887/42671
 

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