Image analysis for gene expression based phenotype characterization in yeast cells

Leiden Repository

Image analysis for gene expression based phenotype characterization in yeast cells

Type: Doctoral Thesis
Title: Image analysis for gene expression based phenotype characterization in yeast cells
Author: Tleis, M.
Issue Date: 2016-07-06
Keywords: Image analysis
Phenotype characterization
Gene expression
Yeast
Saccharomyces cerevisiae
Segmentation
Abstract: Image analysis of objects in the microscope scale requires accuracy so that measurements can be used to differentiate between groups of objects that are being studied. This thesis deals with measurements in yeast biology that are obtained through microscope images. We study the algorithms and workflow of image analysis of yeast cells in order to understand and improve the measurement accuracy. The Saccharomyces cerevisiae cell is widely used as a model organism in the life sciences. It is essential to study the gene and protein behaviour within these cells, and consequently making it possible to find treatment and solutions for genetic and hereditary diseases. This is possible since many processes that occurs at the molecular level in this organism are similar to those in human cells. In the research group Imaging and Bioinformatics, we have developed a framework for analysis of yeast cells. This framework is intended to serve as a support for research in yeast biology. The framework is integrated in one application and presented via a GUI. The application integrates modules and algorithms including segmentation, measurement, analysis and visualization.
Promotor: Supervisors: T.H.W. Bäck; A. Plaat Co-Supervisor: F.J. Verbeek
Faculty: Science
University: Leiden
Uri: urn:isbn:9789402802368
Handle: http://hdl.handle.net/1887/41480
 

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