Fluorescent gene reporters in human pluripotent stem cells : as model for studying human heart development and cardiomyocyte differentiation

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Fluorescent gene reporters in human pluripotent stem cells : as model for studying human heart development and cardiomyocyte differentiation

Type: Doctoral Thesis
Title: Fluorescent gene reporters in human pluripotent stem cells : as model for studying human heart development and cardiomyocyte differentiation
Author: Hartogh, S.C. den
Issue Date: 2016-05-18
Keywords: Stem Cells
Cardiomyocytes
Human, Pluripotent
Fluorescent reporter
Differentiation
Cell Culture
Abstract: It is critical to gain knowledge in the underlying mechanisms that control human cardiovascular developm ent, which helps us to understand the onset of congenital cardiovascular diseases, and to develop optimal culture methods for efficient in vitro cardiomyocyte differentiation from hPSCs, which are of interest for final translational applications including screening and efficacy assays for disease modelling, drug discovery and development, personalized medicine, and perhaps the regeneration of cardiovascular tissues for therapeutic purposes. In this thesis, we show how genetic manipulation of human pluripotent stem cells (hPSCs), resulting in the genomic integration of a fluorescent protein encoding sequence at the locus of a key cardiac transcription factor, allows us to visualize and isolate early pre-cardiac progenitors subpopulations, and to study the molecular mechanisms involved in their further differentiation to cells of the cardiac lineage, including smooth muscle cells, endothelial cells, and cardiomyocyte subtypes.
Promotor: Supervisors: C.L. Mummery; R. Passier
Faculty: Medicine, Leiden University Medical Center (LUMC)
University: Leiden
Uri: urn:isbn:9789462954823
Handle: http://hdl.handle.net/1887/39677
 

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