Sequence dependent mechanics of forced nucleosome unwrapping

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Sequence dependent mechanics of forced nucleosome unwrapping

Type: Master thesis
Title: Sequence dependent mechanics of forced nucleosome unwrapping
Author: Bruin, Lennart de
Issue Date: 2015-09-30
Keywords: dna
unwrapping
sequence-dependence
force
601
nucleosome
rigid base pair
simulation
Monte Carlo
Abstract: Accessibility to nucleosomal dna is an important factor in transcription and gene expression. During transcription, rna polymerase exerts a force on the nucleosome under which the nucleosome unwraps, and, as recently shown experimentally, this can happen asymmetrically. In this thesis, we show, using computer simulations of sequence-dependent coarse-grained dna, what causes this asymmetry. We will also show a proof of concept that we can design dna sequences that unwrap in a predetermined way.
Supervisor: Schiessel, Helmut
Faculty: Faculty of Science
Department: Physics (Master)
Specialisation: Theoretical Physics
ECTS Credits: 15
Handle: http://hdl.handle.net/1887/37071
 

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