||Accessibility to nucleosomal dna is an important factor in transcription and gene expression. During transcription, rna polymerase exerts a force on the nucleosome under which the nucleosome unwraps, and, as recently shown experimentally, this can happen asymmetrically. In this thesis, we show, using computer simulations of sequence-dependent coarse-grained
dna, what causes this asymmetry. We will also show a proof of concept that we can design dna sequences that unwrap in a predetermined way.