Cellular models and viral vectors for skeletal and cardiac muscle research

Leiden Repository

Cellular models and viral vectors for skeletal and cardiac muscle research

Title: Cellular models and viral vectors for skeletal and cardiac muscle research
Author: Neshati, Zeinab
Publisher: Department of Molecular Cell Biology and Department of Cardiology, Faculty of Medicine, Leiden University Medical Center (LUMC), Leiden University
Issue Date: 2014-12-23
Keywords: Cell-cell fusion
Muscular dystrophy
Cardiac fibrosis
Cardiac hypertrophy
Cardiac arrhythmia
Atrial fibrillation
Abstract: Skeletal and cardiac muscle disorders are associated with substantial morbidity and mortality. Despite many improvements in the medical and surgical management of these disorders, development of effective treatments has proven to be challenging. This is because of the limited suitability of existing experimental models for acquiring a thorough understanding of the mechanisms underlying skeletal and cardiac muscle diseases and the lack of efficiency and specificity of many of the currently available therapeutic interventions. Therefore, the aim of this thesis was to establish and use dedicated cellular models in combination with genetic interventions to study the biology of skeletal and cardiac muscles in healthy and diseased states and thereby identify potential targets for future therapeutic interventions. The experiments described in this thesis indeed emphasized the importance of cellular models for (i) elucidating the mechanisms underlying skeletal and cardiac muscle diseases and (ii) identification of novel therapeutic targets. This thesis also underlined the usefulness of viral vector-mediated gene transfer technology for development of biological assays and the evaluation of therapeutic targets. In conclusion, application of in vitro models in combination with genetic interventions can improve our understanding of skeletal and cardiac muscle diseases and aid development of new therapies for these disorders.
Description: Promotor: M.J. Schalij, Co-Promotores: A.A.F. de Vries, D.A. Pijnappels
With Summary in Dutch
Faculty: LUMC
Citation: Neshati, Z., 2014, Doctoral Thesis, Leiden University
ISBN: 9789090287089
Handle: http://hdl.handle.net/1887/30224
 

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