High-throughput profiling of small molecules using mass spectrometry

Leiden Repository

High-throughput profiling of small molecules using mass spectrometry

Title: High-throughput profiling of small molecules using mass spectrometry
Author: Raterink, Robert-Jan
Publisher: Leiden Academic Centre for Drug Research (LACDR), Faculty of Science, Leiden University
Issue Date: 2014-10-01
Keywords: Sample pretreatment
Micro-liquid-liquid-extraction
Electroextraction
Microsampling
Metabolomics
Nanoelectrospray
Zebrafish
Bioanalysis
Abstract: With the persistent efforts to improve healthcare while the costs are ever-increasing, there is a growing demand for healthcare innovations including faster and cheaper analytical methods to support clinical decisions. The in-depth phenotyping of a biological system, such as comprehensive small-molecule profiling, will be key for breakthroughs in biomedical and pharmacological research towards the development of personalized medicine. In this thesis the development of new methods towards high-throughput, mass spectrometry-based profiling of small molecules in complex biological samples is presented. One option for high-throughput mass spectrometric profiling is direct infusion in which no separation techniques are employed prior to MS detection. With the pre-analytical part being a major bottleneck in a typical direct-infusion MS-based analytical workflow, the focus was set on the development of new sample pretreatment and sampling procedures, including miniaturized and automated concepts with the potential for high-throughput application.
Description: Promotor: T. Hankemeier, Co-Promotor: R.J. Vreeken
With Summary in Dutch
Faculty: Faculteit der Wiskunde en Natuurwetenschappen
Citation: Raterink, R.J., 2014, Doctoral Thesis, Leiden University
ISBN: 9789074538862
Handle: http://hdl.handle.net/1887/28970
 

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