Single-electrolyte isotachophoresis : on-chip analyte focusing and separation

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Single-electrolyte isotachophoresis : on-chip analyte focusing and separation

Title: Single-electrolyte isotachophoresis : on-chip analyte focusing and separation
Author: Quist, Johannis Willem
Publisher: Leiden Academic Center for Drugs Research, Department Analytical Biosciences, Faculty of Science, Leiden University
Issue Date: 2014-03-20
Keywords: Microvalves
Chip technology
Electric field gradient focusing
Concentration polarization
Abstract: The unification of isotachophoresis (ITP) and electric field gradient focusing (EFGF) is proposed, based on theoretical considerations and experimental evidence from previous literature. This unification increases the versatility of each EFGF method. A novel isotachophoretic separation method is developed which is based on one of the EFGF techniques, namely micro/nanofluidic concentration polarization devices, This novel method, coined depletion zone isotachophoresis (dzITP), requires a single electrolyte only, which is a simplification over conventional isotachophoresis. Moreover, positioning of focused analyte zones, selective release of individual analyte zones and continuous filtering based on ionic mobility differences is possible. Furthermore, it is shown that microvalves in PDMS chip devices can act as nanochannels upon closure, giving rise to a wide range of concentration polarization phenomena. Compounds can be concentrated over 1000-fold and subsequently be released. These techniques give novel possibilities for bioassays and sample preparation.
Description: Promotor: Thomas Hankemeier, Co-promotores: Heiko van der Linden, Paul Vulto
With summary in Dutch
Faculty: Faculteit der Wiskunde en Natuurwetenschappen
Citation: Quist, J.W., 2014, Doctoral thesis, Leiden University
ISBN: 9789074538831
Sponsor: Netherlands Metabolomics Centre (NMC) which is a part of The Netherlands Genomics Initiative/Netherlands Organization for Scientific Research.

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application/pdf Chapter 1 General introduction 155.1Kb View/Open
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application/pdf Acknowledgements_Curriculum Vitae_Publication list 125.7Kb View/Open
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