Environmental and metabolomic study of antibiotic production by actinomycetes

Leiden Repository

Environmental and metabolomic study of antibiotic production by actinomycetes

Title: Environmental and metabolomic study of antibiotic production by actinomycetes
Author: Zhu, Hua
Publisher: Molecular Biotechnology, Institute of Biology (IBL), Faculty of Science, Leiden University
Issue Date: 2014-01-09
Keywords: Actinomycetes
Cryptic antibiotics
Culture conditions
NMR-base metabolomics
Multivariate analysis
Volitile organic compounds
Antibiotic activity
Abstract: This thesis may be regarded as a concept work, to see how feasible drug discovery approaches still are. For this, a strain collection was built up consisting of actinomycetes from soil in the Qinling and Himalaya mountains, which were subsequently tested for antibiotic production against multi-drug resistant clinical isolates. This resulted in close to 100 strains that showed strong antimicrobial activity, which were then analyzed in more detail. Two of the strains were subjected to extensive NMR-based metabolomics assisted by mass spectrometry, and several known and also novel antimicrobial compounds were elucidated. Finally, we also focused on the antibiotic activity of volatile organic compounds (VOCs)
Description: Promotor: G.P. van Wezel
With summary in Dutch
Faculty: Faculteit der Wiskunde en Natuurwetenschappen
Citation: Zhu, H., 2014, Doctoral thesis, Leiden University
Handle: http://hdl.handle.net/1887/22976

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application/pdf Title page_Table of contents 263.3Kb View/Open
application/pdf Chapter 1 General introduction 377.0Kb View/Open
application/pdf Chapter 2 565.6Kb View/Open Full text at publisher site
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application/pdf Chapter 4 1.901Mb View/Open
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application/pdf Chapter 8 General discussion 928.3Kb View/Open
application/pdf Summary in Dutch 215.9Kb View/Open
application/pdf References 469.3Kb View/Open
application/pdf Curriculum Vitae_List of publications 185.9Kb View/Open
application/pdf Propositions 150.4Kb View/Open

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