Nanofluidic tools for bioanalysis : the large advantages of the nano-scale

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Nanofluidic tools for bioanalysis : the large advantages of the nano-scale

Title: Nanofluidic tools for bioanalysis : the large advantages of the nano-scale
Author: Janssen, Kjeld Gertrudus Hendrikus
Publisher: Leiden Amsterdam Center for Drug Research (LACDR), Faculty of Science, Leiden University
Issue Date: 2013-12-19
Keywords: SERSOR
Lab-on-a-Chip
Nanofluidics
SERS
Debye
Electrochemical doublelayer
Siliconoxide
Electrocavitation
Isotachophoresis
Picoliter
Concentration polarization
Microfluidics
Metabolomics
Surface enhanced Raman
Depletion zone
Nanochannel
Nanochannels
Abstract: In this thesis, nanochannels as well as nanofluidic phenomena are used to provide new and miniaturized bioanalytical tools for the life sciences. Isotachophoresis performed in nanochannels showed the focusing and separation of analytes in a 0.4 picoliter volume, which is a volume in the order of a sample from a single cell. Depletion zone isotachophoresis (dzITP) is demonstrated which uses a nanofluidic phenomenon, concentration polarization, to enable isotachophoresis in a microchannel while using only a single electrolyte. A concept for surface enhanced Raman spectroscopy (SERS) sensor (SERSOR) is explored; a coating protecting the SERS surface from irreversible adsorption may enable dynamic measurements of biomolecules in solution in minute volumes. Working with nanochannels has led to the discovery of new unexpected fundamental phenomena: the very high surface to volume ratio in nanochannels causes acidification of solutions introduced in them, despite the presence of up to 1 mol/L of buffer. Also, extreme pressures of more than a 1000 bar may be induced in a nanochannel by what we named electrocavitation, an effects shown to impose a limit on further downscaling of ITP.
Description: Promotores: Thomas Hankemeier, Jan C.T. Eijkel, Co-promotor: Heiko J. van der linden
With summary in Dutch
Faculty: Faculteit der Wiskunde en Natuurwetenschappen
Citation: Jansen, K.G.H., 2013, Doctoral thesis, Leiden University
ISBN: 9789074538800
Sponsor: This research has been financially supported by NanoNed and the Netherlands Metabolomics Centre.
Handle: http://hdl.handle.net/1887/22946
 

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