Classical and paramagnetic NMR spectroscopy techniques applied to different protein systems

Leiden Repository

Classical and paramagnetic NMR spectroscopy techniques applied to different protein systems

Title: Classical and paramagnetic NMR spectroscopy techniques applied to different protein systems
Author: Skinner, Simon Peter
Publisher: Protein Chemistry Group, Leiden Institute of Chemistry (LIC), Faculty of Science, Leiden University
Issue Date: 2013-12-05
Keywords: Paramagnetic
NMR
Cytochrome
P450cam
T4 Lysozyme
Avr2
EiAPR
Abstract: This thesis describes the application of a variety of different classical and paramagnetic NMR to different protein system. To this end cytochrome P450cam was assigned using classical techniques and paramagnetic NMR was used to investigate the solution state of this enzyme in complex with its binding partner, putidaredoxin. The solution structure of Avr2 was also solved using classical NMR spectroscopy techniques. Additionally, an algorithm for the assignment of protein nuclei on the basis pseudocontact shifts, PARAssign, is presented. An investigation of the dynamic behaviour of a paramagnetic tag attached to T4 lysozyme using relaxation dispersion is described.
Description: Promotor: M. Ubbink
With Summary in Dutch
Faculty: Faculteit der Wiskunde en Natuurwetenschappen
Citation: Skinner, S.P., 2013, Doctoral Thesis, Leiden University
ISBN: 9789462034778
Sponsor: NWO Vici grant 700.58.441
Handle: http://hdl.handle.net/1887/22714
 

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