A breached barrier : analysis of stratum corneum lipids and their role in eczematous patients

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A breached barrier : analysis of stratum corneum lipids and their role in eczematous patients

Title: A breached barrier : analysis of stratum corneum lipids and their role in eczematous patients
Author: Smeden, Jeroen van
Publisher: Leiden Academic Center for Drug Research (LACDR), Faculty of Science, Leiden University
Issue Date: 2013-06-20
Keywords: Stratum corneum lipids
Ceramides
Free fatty acids
Lipid organization
Atopic Eczema
Human skin equivalents
Netherton Syndrome
Liquid chromatography / Mass spectrometry
Skin barrier function
Abstract: The stratum corneum is the outermost layer of the skin, and acts as the primary barrier against penetration of pathogens, allergens and other exogenous substances into the lower layers of the skin. Crucial for a proper barrier function are the lipids in the stratum corneum, mainly consisting of ceramides, free fatty acids, and cholesterol. These lipids are highly ordered stacked layers. In atopic eczema the skin barrier is impaired, but the exact role of the lipids is still uncertain. This thesis describes the importance of a proper composition and ordering of the stratum corneum lipids and their role in eczematous patients (atopic eczema and Netherton disease). The results demonstrate that patients suffering from a impaired skin barrier also show different lipid profile. In particular the carbon chain length of the lipids is notably reduced in these eczematous patients. Consequently, the stratum corneum lipids show a less ordered lipid arangement. This is the first time that a strong correlation is observed between the lipid composition, the lipid organization, and the skin barrier function. These studies therefore provide new targets for possible drug therapy of eczematous patients.
Description: Promotor: J.A. Bouwstra, Co-Promotor: R.J. Vreeken
With Summary in Dutch
Faculty: Faculteit der Wiskunde en Natuurwetenschappen
Citation: Smeden, J. van, 2013, Doctoral Thesis, Leiden University
Sponsor: STW, Astellas, Evonik, RiverD, Unilever
Handle: http://hdl.handle.net/1887/20998
 

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