New electromigration-driven enrichment techniques for peptidomics and metabolomics

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New electromigration-driven enrichment techniques for peptidomics and metabolomics

Title: New electromigration-driven enrichment techniques for peptidomics and metabolomics
Author: Lindenburg, Petrus Wilhelmus
Publisher: Division of Analytical Biosciences / Leiden/Amsterdam Center for Drug Research (LACDR), Faculty of Science, Leiden University
Issue Date: 2012-06-05
Keywords: Analytical chemistry
Capillary electrophoresis
Electroextraction
Electromigration
Isotachophoresis
Liquid chromatography
Mass spectrometry
Abstract: In thesis the power of electrophoretic on-line concentration procedures for peptidomics and metabolomics is demonstrated. Tow examples are given: isotachophoresis with spacer molecules and electroextraction. Electromigration is capable of enhancing selective analyte migration. Selective analyte migration can be used to increase the amount of analyte that is injected into an analytical system, while it leaves the sample volume with the contaminants behind. In consequence, the injection of contaminants and bulk components that may disturb sample pretreatment, separation and detection can be avoided and LOD values can be lowered without the problems associated with direct injection of larger sample volumes. The work that is presented in this thesis is focused on fast electrophoresis-based LOD improvement of the analysis of peptides and/or metabolites in plasma and urine.
Description: Promotor: Thomas Hankemeier
With summary in Dutch
Faculty: Faculteit der Wiskunde en Natuurwetenschappen
Citation: Lindenburg, P.W., 2012, Doctoral thesis, Leiden University
ISBN: 9789074538787
Sponsor: TNO Quality of Life
Handle: http://hdl.handle.net/1887/19049
 

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