Shedding light on surface-assembled photosynthetic systems

Leiden Repository

Shedding light on surface-assembled photosynthetic systems

Title: Shedding light on surface-assembled photosynthetic systems
Author: Magis, Johan Gerhard
Publisher: Division Biophysics, Leiden Institute of Physics (LION), Faculty of Science, Leiden University
Issue Date: 2012-05-01
Keywords: Bioelectronics
Cysteine,
Electrode
Energy
LH1
LH2
Light-harvesting
Photocurren
Photosynthesis
RC
Abstract: The interfacing of biomolecules to nanostructures, electrode surfaces and/or optical components constitutes the new discipline of bioelectronics. It is based on electron transfer between a protein and an electrode, and can be monitored by amperometric techniques. The integration of biomolecules with electronics has strong potential for applications in a variety of functional devices, ranging from biosensors to solar cells. In this thesis we explore the possibilities of constructing a bio-electronic device for solar energy conversion by surface-assembly of photosynthetic pigment-protein complexes on a gold electrode. Optical excitation of the photosynthetic pigments gives rise to charge separation in the so-called reaction center complex. Energy conversion is completed by subsequent electron transfer to the electrode, generating a light-induced electric current. The data shows that light-harvesting complexes can be immobilized directly on a gold surface and on various SAM surfaces whilst retaining their full optical functionality. All energy transferring reactions still take place and are similar to those observed for the detergent-solubilized proteins. Furthermore it is shown that LH1 complexes exhibit a remarkable photostability, even under ambient conditions. These findings demonstrate the possibility of interfacing a fully functional energy transferring protein complex to a conducting substrate in the presence of oxygen, with the capacity of converting light into electrical energy.
Description: Promotor: T.J. Aartsma, Co-promotor: R.N. Frese
With summary in Dutch
Faculty: Faculteit der Wiskunde en Natuurwetenschappen
Citation: Magis, J.G., 2012, Doctoral thesis, Leiden University
Series/Report no.: Casimir PhD series;2012-1
ISBN: 9789085931126
Handle: http://hdl.handle.net/1887/18810
 

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