Infectious disease studies in zebrafish : the fish pathogen Edwardsiella tarda as a model system

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Infectious disease studies in zebrafish : the fish pathogen Edwardsiella tarda as a model system

Title: Infectious disease studies in zebrafish : the fish pathogen Edwardsiella tarda as a model system
Author: Soest, Joost Jeremy van
Publisher: Department of Molecular Microbiology and Department of Molecular Cell Biology, Institute of Biology Leiden (IBL), Faculty of Science, Leiden University
Issue Date: 2011-11-29
Keywords: myd88
Edwardsiella tarda
Innate Immunity
Static Immersion
Zebrafish embryo
Infection
Microarray
Deep sequencing technology
Abstract: In a world where bacterial infections are becoming harder and harder to fight due to rising antibiotic resistance, disease models that allow high throughput screening are needed more than ever. An ideal model organism for high throughput screening is the zebrafish embryo. However, models for high throughput screening of infection are still wanted. In this thesis the fish pathogen Edwardsiella tarda was tested for use in high throughput studies of infectious disease in zebrafish. First the genome of Edwardsiella tarda was sequenced to determine the virulence factors of this bacterium. Next different types of exposure of zebrafish to Edwardsiella tarda were tested. This showed an absence of detectable infection after static immersion, but a reproducible infection on the level of single embryos after caudal vein injection. Finally, using the caudal vein injection method, the zebrafish knock-out mutant in the gene Myd88 was characterized. This is the first zebrafish knock-out mutant in a gene central in the innate immune system.
Description: Promotores: H.P. Spaink, C.A.M.J.J. van den Hondel, Co-Promotor: A.H. Meijer
Faculty: Faculteit der Wiskunde en Natuurwetenschappen
Citation: Soest, J.J. van, 2011, Doctoral Thesis, Leiden University
ISBN: 9789053354889
Sponsor: ZF-screens BV
Handle: http://hdl.handle.net/1887/18149
 

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