Dynamic system-wide mass spectrometry based metabolomics approach for a new Era in drug research

Leiden Repository

Dynamic system-wide mass spectrometry based metabolomics approach for a new Era in drug research

Type: Doctoral Thesis
Title: Dynamic system-wide mass spectrometry based metabolomics approach for a new Era in drug research
Author: Castro Perez, Jose Miguel
Publisher: Faculty of Science, Leiden University
Issue Date: 2011-10-18
Keywords: Atherosclerosis
Bile acids
Flux
Lipidomics
Lipids
Mass Spectrometry
Tracers
Transcriptomics
Abstract: The aim of the thesis was to develop metabolic analytical platforms for static and dynamic measurements that could answer biological questions for in vitro and in vivo animal models in the area of lipid research. Gene profiling together with the transcriptome and metabolome data was used in combination with the LC/MS analytical platform. In terms of the analytical platforms developed, the focus was on high resolution LC/MS but not limited, as amalgamation with other platforms such as gradient gel electrophoresis (GGE) and fast protein liquid chromatography (FPLC) were explored in more detail to investigate the lipid composition of lipoprotein particles. These analytical strategies were applied to different lipid modulating biological targets as a mean to obtaining a more detailed and characteristic phenotype description directing decisions in drug search during the drug discovery process on the basis of the analytical results obtained. Additionally, the utilization of metabolic tracers was investigated further to probe dynamic changes in the biological target and animal models in question
Description: Promotor: T. Hankemeier, Co-promotores: R.J. Vreeken, T.P. Roddy
With summary in Dutch
Faculty: Faculteit der Wiskunde en Natuurwetenschappen
Citation: Castro Perez, J.M., 2011, Doctoral thesis, Leiden University
ISBN: 9789074538763
Handle: http://hdl.handle.net/1887/17954
 

Files in this item

Description Size View
application/pdf Full text 5.715Mb View/Open
application/pdf Cover 148.1Kb View/Open
application/pdf Title page_Table of contents 200.8Kb View/Open
application/pdf Chapter 1 General introduction and scope 1.133Mb View/Open
application/pdf Part I Chapter 2 1.466Mb View/Open Full text at publisher site
application/pdf Part I Chapter 3 707.4Kb View/Open Full text at publisher site
application/pdf Part II Chapter 4 405.5Kb View/Open
application/pdf Part II Chapter 5 907.0Kb View/Open Full text at publisher site
application/pdf Part II Chapter 6 701.1Kb View/Open
application/pdf Part III Chapter 7 656.8Kb View/Open Full text at publisher site
application/pdf Part III Chapter 8 499.4Kb View/Open Full text at publisher site
application/pdf Part III Chapter 9 1.306Mb View/Open Full text at publisher site
application/pdf Chapter 10 Summary and future perspectives 208.9Kb View/Open
application/pdf Appendix_Summar ... lum Vitae_Acknowledgements 197.8Kb View/Open
application/pdf Propositions 91.58Kb View/Open

This item appears in the following Collection(s)