The zebrafish as a model for tissue regeneration and bone remodelling

Leiden Repository

The zebrafish as a model for tissue regeneration and bone remodelling

Title: The zebrafish as a model for tissue regeneration and bone remodelling
Author: Sharif, Faiza
Publisher: Department of Integrative Zoology, Institute of Biology (IBL), Faculty of Science, Leiden University
Issue Date: 2011-10-12
Keywords: Bone remodelling
Embryos
Macrophages
Mesoporous silica nanoparticles.
Neutrophils
Osteoclasts
Regeneration
Zebrafish
Zebrafish scales
Abstract: The aim of this thesis was to investigate the expression, and function of genes associated with remodelling and regeneration in the zebrafish model species. Here, we studied the role of cell populations, defined by their expression of markers, in bone regeneration and remodelling in zebrafish embryos and adult zebrafish scales. We used mesoporous silica nanoparticles to carry cytokines, known to activate hematopoietic cells into osteoclasts, into tissues of the living embryo. We further investigated the role of genes such as matrix metalloproteinases in regeneration of adult zebrafish scales. These genes are known to be involved in matrix degradation and have been found to be expressed in mammalian osteoclasts. We studied both by in situ hybridisation and immunocytochemistry the presence of mononucleated and multinucleated mmp-9 positive cells on the episquamal side of adult zebrafish scales. Finally we studied the regeneration of the caudal fin of zebrafish embryos with a special emphasis on the effect of glucocorticoids on regeneration and wound healing. Glucocorticoids in this case mimic stress conditions in the embryos, thus helping understand the effect of early exposure to stress on wound healing and tissue Remodelling.
Description: Promotor: Michael K. Richardson
With summary in Dutch
Faculty: Faculteit der Wiskunde en Natuurwetenschappen
Citation: Sharif, F., 2011, Doctoral thesis, Leiden University
Sponsor: Higher Education Commission of Pakistan and the Smart Mix Programme of the Netherlands Ministry of Economic Affairs and The Netherlands Ministry of Education, Culture and Science.
Handle: http://hdl.handle.net/1887/17923
 

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application/pdf Chapter 1 Introduction 160.3Kb View/Open
application/pdf Chapter 2 2.654Mb View/Open
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application/pdf Chapter 5 2.321Mb View/Open
application/pdf Chapter 6 Summary and discussion 104.6Kb View/Open
application/pdf References 215.7Kb View/Open
application/pdf Summary in Dutch 63.11Kb View/Open
application/pdf Acknowledgement ... contributions_Publications 97.89Kb View/Open
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