VirD2 of Agrobacterium tumefaciens : functional domains and biotechnological applications

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VirD2 of Agrobacterium tumefaciens : functional domains and biotechnological applications

Type: Doctoral Thesis
Title: VirD2 of Agrobacterium tumefaciens : functional domains and biotechnological applications
Author: Kregten, Maartje van
Publisher: Molecular and Development Genetics, Institute Biology Leiden, Faculty of Science, Leiden University
Issue Date: 2011-05-19
Keywords: Agrobacterium tumefaciens
Arabidopsis thaliana
relaxase
protein translocation
Type 4 Secretion System
Abstract: Agrobacterium tumefaciens is a pathogenic bacterium, which can genetically transform plants and other organisms. It does so by translocating a part of its DNA, the T-strand, in complex with the relaxase protein VirD2. We have shown that VirD2 is the determinant of translocation of the VirD2-T-strand complex, rather than the T-strand. Furthermore, we have elucidated the role of the DUF domain of VirD2. DUF is shown to be important for localization of VirD2 to the cell poles. Polar localization is essential for translocation of VirD2 to the recipient cell. Using VirD2 as a carrier, we have shown that several biotechnological important enzymes can be transferred to Arabidopsis thaliana. Of the relaxase-homing endonuclease fusion protein VirD2-I-SceI, we have shown that it can induce the formation of double-strand breaks in the Arabidopsis genome after translocation from Agrobacterium.
Description: Promotor: P.J.J. Hooykaas, Co-promotor: E.J. van der Zaal
With summary in Dutch
Faculty: Faculteit der Wiskunde en Natuurwetenschappen
Citation: Kregten, M. van, 2011, Doctoral thesis, Leiden University
ISBN: 9789085707470
Handle: http://hdl.handle.net/1887/17648
 

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