Spatial and dynamic organization of molecular structures in the cell nucleus

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Spatial and dynamic organization of molecular structures in the cell nucleus

Type: Doctoral Thesis
Title: Spatial and dynamic organization of molecular structures in the cell nucleus
Author: Brouwer, Anne-Kee
Publisher: Department Molecular Cell Biology, Faculty of Medicine / Leiden University Medical Center (LUMC), Leiden University
Issue Date: 2010-09-08
Keywords: Chromatin
DNA damage
Live-cell imaging
MMS
Nuclear bodies
Nuclear lamina
Nuclear matrix
Nuclear organization
PML bodies
Telomeres
Abstract: In this thesis we attempt to provide a better understanding of the principles that underlie the spatial dynamic organization of the cell nucleus. Chapter 1 reviews the current status of knowledge about the structural and functional organization of the cell nucleus. In chapter 2, the development of a computer program is described that has been designed to track the 2D and 3D motion of objects in the nucleus of living cells. In chapter 3, evi-dence is provided for the existence of a nuclear matrix structure that is composed of lamin proteins, emerin and actin. By analyzing the dynamics of telomeres in nuclei of cells showing reduced levels of lamin expression, it is investigated whether telomeres anchor to an inner nuclear lamina structure. In chapter 4 the de novo formation of PML nuclear bodies is described. Using live cell imaging and immunocytochemistry it is dem-onstrated that telomeres play a role in the de novo formation of PML bodies. In chapter 5 it is investigated whether nuclear bodies are associated with chromatin in the cell nucleus. After treating cells with DNA alkylating agent MMS, the dynamics of PML bodies, Cajal bodies and speckles has been analyzed relative to chromatin in the 3D space of the cell nucleus
Description: Promotor: H.J. Tanke, Co-promotor: R.W. Dirks
With summary in Dutch
Faculty: LUMC
Citation: Brouwer, A.-K., 2010, Doctoral thesis, Leiden University
Sponsor: Jurriaanse Stichting
Handle: http://hdl.handle.net/1887/15930
 

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application/pdf Acknowledgements 19.37Kb View/Open
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