NMR-based metabolomic characterization of Vanilla planifolia

Leiden Repository

NMR-based metabolomic characterization of Vanilla planifolia

Type: Doctoral Thesis
Title: NMR-based metabolomic characterization of Vanilla planifolia
Author: Palama, Tony Lionel
Publisher: Department of Pharmacognosy, Institute of Biology, Faculty of Science, Leiden University
Issue Date: 2010-06-10
Keywords: Vanilla planifolia
Orchidaceae
CAM plants
Vanillin
Glucovanillin
Nuclear magnetic resonance
Phenolic glycosides
Pod development
Callus
Metabolomic analysis
Nuclear magnetic resonance
Abstract: Vanilla planifolia, a flowering epiphytic orchid, is the major natural source of vanilla flavour. Largely used in dairy products, beverages, bakeries and perfume, vanilla flavour is obtained after a long process: from eight to nine months after flower pollination, mature pods are harvested and then prepared during about one year in order to release the characteristic vanilla aroma. Nowadays, more than half of vanilla pods world production comes from Madagascar. To face the concurrence, a solution could be to develop higher quality pods. Selection of the most aromatic vanilla plant is then preferred. Nevertheless, amelioration program are facing up to a lack of knowledge in vanilla plant physiology. It is now essential to understand more the physiological and biochemical mechanisms implied in the aromatic production of V. planifolia pods. In this thesis, a metabolomic analysis of vanilla green pods and leaves has been performed by nuclear magnetic resonance. This technique has allowed the qualitative and quantitative analysis of primary (sugar, amino and organic acids…) and secondary metabolites (phenolic compounds…) present in vanilla plant according to various physiological conditions: developing pods, viral infection, inter-accession or seasonal variation.
Description: Promotores: R. Verpoorte, H. Kodja, Co-promotor: Y.H.Choi
With summaries in Dutch and French
Faculty: Faculteit der Wiskunde en Natuurwetenschappen
Citation: Palama, T.L., 2010, Doctoral Thesis, Leiden University
Sponsor: the Regional Council of La Réunion and the European Social Fund
Handle: http://hdl.handle.net/1887/15674
 

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application/pdf Title pages_Contents 192.4Kb View/Open
application/pdf Chapter 1 171.7Kb View/Open
application/pdf Chapter 2 4.233Mb View/Open
application/pdf Chapter 3 505.8Kb Under embargo Full text at publisher site
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application/pdf Chapter 8 226.3Kb View/Open
application/pdf References 216.8Kb View/Open
application/pdf Summary in English 31.86Kb View/Open
application/pdf Summary in Dutch 38.12Kb View/Open
application/pdf Summary in French 33.14Kb View/Open
application/pdf Curriculum Vitae_ Publications_Acknowledgements 145.8Kb View/Open
application/pdf Propositions 177.5Kb View/Open

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