Cancer and inflammation studies using zebrafish cell lines

Leiden Repository

Cancer and inflammation studies using zebrafish cell lines

Type: Doctoral Thesis
Title: Cancer and inflammation studies using zebrafish cell lines
Author: He, Shuning
Publisher: Institute of Biology (IBL), Faculty of Science, Leiden University
Issue Date: 2010-05-27
Keywords: Allo-transplantation
Hepatocellular carcinoma
Kinomics
MAPK signaling
PAC2
TLR signaling
Transcriptomics
Zebrafish
ZF4
ZFL
Abstract: As the zebrafish, Danio rerio, has been increasingly used as an animal model for biomedical research, we aimed to establish zebrafish cell line models for inflammation and cancer studies in this thesis. Several zebrafish cell lines were characterized and their genetic and physiological properties were compared. We also developed a set of tool methods to investigate cellular signaling events in zebrafish cell lines. Our case studies illustrated that zebrafish cell lines are as reliable models as the widely used mammalian cell cultures. Taking advantage of the transparency of zebrafish embryos and cell implantation protocols, zebrafish cell lines can serve as a bridge platform between in vitro, in silico, ex vivo and in vivo studies in order to enhance our understanding of molecular mechanisms underlying disease progression.
Description: Promotor: H.P. Spaink, Co-promotor: B.E. Snaar-Jagalska
With summary in Dutch
Faculty: Faculteit der Wiskunde en Natuurwetenschappen
Citation: He, S., 2010, Doctoral thesis, Leiden University
ISBN: 9789085705659
Handle: http://hdl.handle.net/1887/15555
 

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